DCR Public Strain Repository

syb7849

unc-47::gfp11x3 (syb7849) 2X outcrossed

sybIs9614

Peft-3::FLP - single copy 2X outcrossed Pan-cellular expression of flippase - single copy in chromosome II, landing site ttTi5605

egxIs136

egxSi136 [mex-5p::tomm-20::halotag::pie-1 3’UTR + unc-119(+)] II. Superficially wild-type. Stable expression of TOMM-20 tagged with Halo on the outer membranes of mitochondria in the germline and embryos. Reference: Fan X, et al. G3 (accepted).

sybIs9709

SybIs9709. Single copy MOSCI insertion of pceh-48: Flippase in chromosome ii. Made by SUNY. SUNY alias DCR45/PHX9709. Insertion of Pceh-48-SL2-SV40 NLS-FLP-egl-13 NLS-let-858 3’UT into chromosome ii. Parent Background strain: (this is the strain SUNY uses for mosci intertions on II) PHX2055 sybIs2055, which has a sgRNA target site inserted in ttTi5605 (ch ii) MosI site on N2,

syb9139

eat-4::mRuby FLP-on (syb9193) III 2X outcrossed Cell-specific mRuby3 knock-in (C-terminus end)

syb8059

unc-17 is endogenously expressing FLP. This strain is two times outcrossed to get rid of any background mutations. Strain was not thaw checked.

syb8125

unc-47 driving expression of FLP. This strain was two times outcrossed to get rid of any background mutations. Strain was not thaw checked.

syb8568

eat-4::GFP FLP-on (syb8568) III 2X outcrossed Cell-specific GFP knock-in (C-terminus end)

syb7358

Endogenous full body mKate2 tagging 2 times outcrossed

syb7882

Cell-specific mRuby3 knock-in (C-terminus end)

syb7313; gfp11

Endogenous and cell-specific GFP labeling

syb7251

Outcrossed strain that labels the endogenous UNC-17 gene at the N-terminus end.

ola503

unc-17::gfp FLP-on (ola503) 1X outcrossed

syb6990

unc-47::GFP knock-in Endogenous full body GFP tagging 2X outcrossed.

syb5949; syb6275

unc-25 cell-specific knockout. This strain has a FRT sequence before the methionine and a FRT+let859+SL2+nuclearmRuby at the C-terminus end. Expression of flippase triggers the elimination of the unc-25 ORF and the activation of nuclear mRuby. Red nuclei labels the cells where GABA production is turned off.